mouse α cpsf7 Search Results


mouse cpsf3/cpsf73 gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse cleavage and polyadenylation specificity factor 3
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hpa041094  (Atlas Antibodies)
90
Atlas Antibodies hpa041094

Hpa041094, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+%CE%B1+cpsf7/Anti-CPSF7/pmc06400501-10-9-6
Average 90 stars, based on 1 article reviews
hpa041094 - by Bioz Stars, 2026-09
90/100 stars
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a 9 against cpsf7  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology a 9 against cpsf7
Figure 6. HIV-1 infection induces the formation of CPSF5 condensates that colocalize with CPSF6. A549 cells were infected or not (mock) with HIV-1-Luc at an MOI of ~ 2 for 24 h (A and B). Cells were fixed, permeabilized, and coimmunostained using rabbit polyclonal antibody to CPSF6 with mouse monoclonal antibody against CPSF5 (A) or with mouse monoclonal antibody against <t>CPSF7</t> (B). Secondary antibodies were Alexa-488-conjugated donkey anti-mouse IgG (green channel) and Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Stained cells were examined by fluorescence microscopy. Merging the red and green channels generated the third image in each row; yellow indicates overlapping localization of the red and green channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.
A 9 Against Cpsf7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+%CE%B1+cpsf7/CPSF7+Antibody/pm37414787-329-23-28
Average 94 stars, based on 1 article reviews
a 9 against cpsf7 - by Bioz Stars, 2026-09
94/100 stars
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anti cpsf7  (Bethyl)
93
Bethyl anti cpsf7
Figure 6. HIV-1 infection induces the formation of CPSF5 condensates that colocalize with CPSF6. A549 cells were infected or not (mock) with HIV-1-Luc at an MOI of ~ 2 for 24 h (A and B). Cells were fixed, permeabilized, and coimmunostained using rabbit polyclonal antibody to CPSF6 with mouse monoclonal antibody against CPSF5 (A) or with mouse monoclonal antibody against <t>CPSF7</t> (B). Secondary antibodies were Alexa-488-conjugated donkey anti-mouse IgG (green channel) and Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Stained cells were examined by fluorescence microscopy. Merging the red and green channels generated the third image in each row; yellow indicates overlapping localization of the red and green channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.
Anti Cpsf7, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+%CE%B1+cpsf7/CPSF59+Antibody/10__1074_slash_jbc__m116__721647-58-32-34
Average 93 stars, based on 1 article reviews
anti cpsf7 - by Bioz Stars, 2026-09
93/100 stars
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1 ap  (Proteintech)
93
Proteintech 1 ap
Figure 6. HIV-1 infection induces the formation of CPSF5 condensates that colocalize with CPSF6. A549 cells were infected or not (mock) with HIV-1-Luc at an MOI of ~ 2 for 24 h (A and B). Cells were fixed, permeabilized, and coimmunostained using rabbit polyclonal antibody to CPSF6 with mouse monoclonal antibody against CPSF5 (A) or with mouse monoclonal antibody against <t>CPSF7</t> (B). Secondary antibodies were Alexa-488-conjugated donkey anti-mouse IgG (green channel) and Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Stained cells were examined by fluorescence microscopy. Merging the red and green channels generated the third image in each row; yellow indicates overlapping localization of the red and green channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+%CE%B1+cpsf7/CPSF7+Antibody/pm37777964-513-28-26
Average 93 stars, based on 1 article reviews
1 ap - by Bioz Stars, 2026-09
93/100 stars
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cpsf7 mouse qpcr primer pair  (OriGene)
N/A
qSTAR qPCR primer pairs against Mus musculus gene Cpsf7
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cpsf7 mouse shrna plasmid  (OriGene)
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Cpsf7 Mouse 4 unique 29mer shRNA constructs in retroviral untagged vector
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mouse cpsf3/cpsf73 gene orf cdna clone expression plasmid, c-ha tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse cleavage and polyadenylation specificity factor 3
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mouse cpsf7 activation kit by crispra  (OriGene)
N/A
Cpsf7 CRISPRa kit CRISPR gene activation of mouse cleavage and polyadenylation specific factor 7
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mouse cpsf3/cpsf73 gene orf cdna clone expression plasmid, n-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse cleavage and polyadenylation specificity factor 3 with N terminal GFPSpark tag.
   Buy from Supplier
mouse cpsf3/cpsf73 gene orf cdna clone expression plasmid, n-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse cleavage and polyadenylation specificity factor 3 with N terminal OFPSpark / RFP tag.
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Image Search Results


Journal: eLife

Article Title: HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex

doi: 10.7554/eLife.41800

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-hCPSF7 , Atlas Antibodies Cat# HPA041094 , RRID: AB_10794187 , IF(1:200).

Techniques: Enzyme-linked Immunosorbent Assay, Imaging, Reverse Transcription, Recombinant, Plasmid Preparation, Expressing, shRNA, Control, Software

Figure 6. HIV-1 infection induces the formation of CPSF5 condensates that colocalize with CPSF6. A549 cells were infected or not (mock) with HIV-1-Luc at an MOI of ~ 2 for 24 h (A and B). Cells were fixed, permeabilized, and coimmunostained using rabbit polyclonal antibody to CPSF6 with mouse monoclonal antibody against CPSF5 (A) or with mouse monoclonal antibody against CPSF7 (B). Secondary antibodies were Alexa-488-conjugated donkey anti-mouse IgG (green channel) and Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Stained cells were examined by fluorescence microscopy. Merging the red and green channels generated the third image in each row; yellow indicates overlapping localization of the red and green channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.

Journal: Scientific reports

Article Title: Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection.

doi: 10.1038/s41598-023-37364-x

Figure Lengend Snippet: Figure 6. HIV-1 infection induces the formation of CPSF5 condensates that colocalize with CPSF6. A549 cells were infected or not (mock) with HIV-1-Luc at an MOI of ~ 2 for 24 h (A and B). Cells were fixed, permeabilized, and coimmunostained using rabbit polyclonal antibody to CPSF6 with mouse monoclonal antibody against CPSF5 (A) or with mouse monoclonal antibody against CPSF7 (B). Secondary antibodies were Alexa-488-conjugated donkey anti-mouse IgG (green channel) and Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Stained cells were examined by fluorescence microscopy. Merging the red and green channels generated the third image in each row; yellow indicates overlapping localization of the red and green channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.

Article Snippet: We used the following mouse monoclonal antibodies: clone SC-35 against SC35 (Cat# ab11826, Abcam), clone 3F8 against CPSF5 (Cat# H00011051-M12, Novus Biologicals), clone A-9 against CPSF7 (Cat# sc-393880, Santa Cruz) and clone F3 against CPSF6 (Cat# sc-376228, Santa Cruz).

Techniques: Infection, Staining, Fluorescence, Microscopy, Generated

Figure 7. HIV-1 infection induces the formation of CPSF6 and CPSF5 condensates in human T cells and human primary macrophages. Jurkat T cells (A) and macrophages (B) were infected or not (mock) with HIV- 1-GFP at an MOI of ~ 2 for 48 or 72 h, respectively. At 48hpi, 2,5 × 105 Jurkat cells were seeded on glass coverslips previously treated with poly-D-lysine for 1 h. Cells were fixed, permeabilized, and immunostained using rabbit polyclonal antibody to CPSF6, mouse monoclonal antibody against CPSF5 or mouse monoclonal antibody against CPSF7. Secondary antibody was Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Nuclei were stained with DAPI (blue channel). Stained cells were examined by fluorescence microscopy. Merging the red and blue channels generated the third image in each row; magenta indicates overlapping of the red and blue channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.

Journal: Scientific reports

Article Title: Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection.

doi: 10.1038/s41598-023-37364-x

Figure Lengend Snippet: Figure 7. HIV-1 infection induces the formation of CPSF6 and CPSF5 condensates in human T cells and human primary macrophages. Jurkat T cells (A) and macrophages (B) were infected or not (mock) with HIV- 1-GFP at an MOI of ~ 2 for 48 or 72 h, respectively. At 48hpi, 2,5 × 105 Jurkat cells were seeded on glass coverslips previously treated with poly-D-lysine for 1 h. Cells were fixed, permeabilized, and immunostained using rabbit polyclonal antibody to CPSF6, mouse monoclonal antibody against CPSF5 or mouse monoclonal antibody against CPSF7. Secondary antibody was Alexa-594-conjugated donkey anti-rabbit IgG (red channel). Nuclei were stained with DAPI (blue channel). Stained cells were examined by fluorescence microscopy. Merging the red and blue channels generated the third image in each row; magenta indicates overlapping of the red and blue channels. Experiments were repeated at least three times, and a representative experiment is shown. Scale bar = 10 μm.

Article Snippet: We used the following mouse monoclonal antibodies: clone SC-35 against SC35 (Cat# ab11826, Abcam), clone 3F8 against CPSF5 (Cat# H00011051-M12, Novus Biologicals), clone A-9 against CPSF7 (Cat# sc-393880, Santa Cruz) and clone F3 against CPSF6 (Cat# sc-376228, Santa Cruz).

Techniques: Infection, Staining, Fluorescence, Microscopy, Generated